主管单位:中华人民共和国
国家卫生健康委员会
主办单位:
总编辑:杨秋
编辑部主任:吴翔宇
邮发代号:80-528
定价:28.00元
全年:336.00元
Email:zgyy8888@163.com
电话(传真):010-64428528;
010-64456116(总编室)
英文作者:Sun Weiluo Liu Suying Li Sijin Zhou Longmei He Peiyuan
英文单位:Department of Gastroenterology Affiliated Hospital of Chengde Medical University Chengde 067000 China
关键词:结肠癌;微小RNA-452;微小RNA-762;预后
英文关键词:Coloncancer;MicroRNA-452;MicroRNA-762;Prognosis
目的 探究结肠癌组织微小RNA(miR)-452、miR-762表达及与患者预后的关系。方法 选取2019年4月至2020年4月承德医学院附属医院收治的90例结肠癌患者作为研究对象,术中取肿瘤组织及癌旁组织,并根据3年随访预后情况分为预后良好组与预后不良组。采用实时荧光定量聚合酶链反应法检测组织中miR-452、miR-762的表达。Pearson相关分析法分析miR-452与miR-762表达的相关性。多因素Logistic回归法分析结肠癌患者预后的影响因素。受试者工作特征曲线分析结肠癌患者肿瘤组织中miR-452、miR-762表达水平对预后的预测价值。结果 结肠癌患者肿瘤组织中miR-452、miR-762表达水平均高于癌旁组织[(2.23±0.24)比(1.00±0.00)、(1.86±0.20)比(1.01±0.13)],差异均有统计学意义(t=48.620、33.805,均P<0.001)。miR-452、miR-762表达水平与患者肿瘤分化程度、TNM分期有关(均P<0.05)。随访3年预后不良的发生率为27.8%(25/90)。预后不良组患者肿瘤组织中miR-452、miR-762表达水平均高于预后良好组[(2.56±0.30)比(2.10±0.22)、(2.15±0.30)比(1.75±0.16)](t=7.997、8.181,均P<0.001)。Pearson相关性分析结果显示,结肠癌患者肿瘤组织中miR-452与miR-762表达水平呈正相关(r=0.560,P<0.001)。多因素Logistic回归分析结果显示,miR-452、miR-762均是结肠癌患者发生不良预后的危险因素(均P<0.05)。miR-452、miR-762联合预测结肠癌患者预后的受试者工作特征曲线下面积优于miR-452单独预测(Z=2.745,P=0.006)。结论 结肠癌患者肿瘤组织中miR-452、miR-762表达上调,其与结肠癌患者预后有关,可能是结肠癌预后评估的生物标志物。
Objective To investigate the expression of microRNA(miR)-452 and miR-762 in colon cancer tissue and their relationship with patients′ prognosis. Methods Ninety patients with colon cancer admitted to Affiliated Hospital of Chengde Medical University from April 2019 to April 2020 were collected as research subjects. Tumor tissue and adjacent tissue were taken during surgery, and the prognosis of the good prognosis group and the poor prognosis group were determined based on the 3-year follow-up. Real-time quantitative polymerase chain reaction was applied to detect the expression of miR-452 and miR-762 in tissues. Pearson correlation analysis was applied to analyze the correlation between miR-452 and miR-762 expression. Multivariate Logistic regression was applied to analyze the prognostic factors of colon cancer patients. Receiver operating characteristic curve was applied to analyze the prognostic value of miR-452 and miR-762 expression levels in tumor tissues of colon cancer patients. Results Compared with adjacent tissues, the expression levels of miR-452 and miR-762 in tumor tissues of colon cancer patients were obviously increased [(2.23±0.24) vs (1.00±0.00), (1.86±0.20) vs (1.01±0.13)](t=48.620, 33.805, both P<0.001). The expression levels of miR-452 and miR-762 were related to TNM staging and tumor differentiation (all P<0.05). The incidence of poor prognosis was 27.8% (25/90) during 3-year follow-up. Compared with the good prognosis group, the expression levels of miR-452 and miR-762 in tumor tissue of patients in the poor prognosis group were obviously increased [(2.56±0.30) vs (2.10±0.22), (2.15±0.30) vs (1.75±0.16)](t=7.997, 8.181, both P<0.001). Pearson correlation analysis showed there was a positive correlation between the expression levels of miR-452 and miR-762 in tumor tissue of colon cancer patients(r=0.560,P<0.001). Multivariate Logistic regression analysis showed that miR-452 and miR-762 were risk factors for poor prognosis in colon cancer patients (both P<0.05). The area under the curve of the ROC of the combination of miR-452 and miR-762 in predicting the colon cancer was better than that of miR-452 alone (Z=2.745, P=0.006). Conclusion The expression of miR-452 and miR-762 is up-regulated in tumor tissues of colon cancer patients, and it is related to the prognosis of colon cancer patients and may be a biomarker for evaluating the prognosis of colon cancer.
copyright
地址:北京市朝阳区安贞路2号首都医科大学附属北京安贞医院北楼二层
电话:010-64456116 传真:010-64428528 邮编:100029 Email: zgyy8888@163.com
网址: 京ICP备2020043099号-3
当您在使用本网站投稿遇到困难时,请直接将稿件投送到编辑部邮箱zgyy8888@163.com。