2022 年第 8 期 第 17 卷

CD147和血小板源性生长因子CC在三阴性乳腺癌细胞中的表达及对细胞功能的影响

Expressions of CD147 and platelet-derived growth factor CC in triple-negative breast cancer cell and their effects on cell function

作者：胡光富1 叶辉2 张凌飞3 汪成1单鸣1陆永伟1牛瑞洁1

英文作者：Hu Guangfu1 Ye Hui2 Zhang Lingfei3 Wang Cheng1 Shan Ming1 Lu Yongwei1 Niu Ruijie1

单位：1上海交通大学医学院附属第九人民医院黄浦分院乳腺外科，上海200011；2上海交通大学医学院附属第九人民医院黄浦分院普外科，上海200011；3中国科学院生物化学与细胞生物学研究所，上海200031

英文单位：1Department of Breast Surgery Huangpu Branch Shanghai Ninth People′s Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200011 China; 2Department of General Surgery Huangpu Branch Shanghai Ninth People′s Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200011 China; 3Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences Shanghai 200031 China

关键词：三阴性乳腺癌；血小板源性生长因子CC；细胞功能

英文关键词：Triple-negativebreastcancer;Platelet-derivedgrowthfactorCC;Cellfunction

• 摘要：

• 目的 探讨CD147和血小板源性生长因子CC（PDGF-CC）在三阴性乳腺癌（TNBC）细胞系MDA-MB-231中的表达及对细胞功能的影响。方法 采用流式细胞仪筛选MDA-MB-231中CD147低表达和CD147高表达2个细胞亚群，采用免疫荧光染色检测2个细胞亚群中CD147蛋白和PDGF-CC蛋白表达情况。通过短发夹RNA干扰技术，将CD147高表达的细胞亚群分为空白对照组、Ctrl RNA组、sh-CD147组、sh-PDGF-CC组、sh-CD147+sh-PDGF-CC组。比较各组细胞增殖活性及细胞迁移功能。结果 CD147高表达细胞亚群中CD147和PDGF-CC蛋白表达水平高于CD147低表达细胞亚群。与空白对照组相比，sh-CD147组、sh-PDGF-CC组及sh-CD147+sh-PDGF-CC组CD147高表达细胞亚群的增殖活性更低，差异均有统计学意义（均P＜0.05），其中sh-CD147+sh-PDGF-CC组细胞增殖活性最低。sh-CD147组、sh-PDGF-CC组、sh-CD147+sh-PDGF-CC组的迁移细胞数量少于空白对照组和Ctrl RNA组［（20.7±4.5）、（13.7±2.5）、（7.3±2.1）个比（50.0±4.6）、（51.3±9.5）个］，差异有统计学意义（F=45.130，P＜0.001），且sh-CD147+sh-PDGF-CC组少于sh-CD147组和sh-PDGF-CC组，差异有统计学意义（F=12.910，P=0.006）。结论 在TNBC细胞系MDA-MB-231细胞中，CD147高表达细胞亚群的PDGF-CC蛋白表达水平也更高，下调CD147和PDGF-CC蛋白的表达可起到协同抑制细胞增殖和细胞迁移功能的作用。

• Objective To explore the expressions of CD147 and platelet-derived growth factor CC (PDGF-CC) in triple-negative breast cancer (TNBC) cell line MDA-MB-231 and their effects on cell function. Methods Flow cytometry was used to distinguish the subcellular lines between low-CD147 expression and high-CD147 expression from MDA-MB-231. Immunofluorescence staining was used to detected expressions of CD147 and PDGF-CC protein in the two subcellular lines. High-CD147 expression subcellular line was divided into Mock group, Ctrl RNA group, sh-CD147 group, sh-PDGF-CC group and sh-CD147+sh-PDGF-CC group according to short hairpin RNA interference technology. The cell proliferation activity and cell migration function were compared among the groups. Results The expressions of CD147 and PDGF-CC protein in high-CD147 expression subcellular line were higher than those in low-CD147 expression subcellular line. Compared with Mock group, the proliferative activity in sh-CD147 group, sh-PDGF-CC group and sh-CD147+sh-PDGF-CC group decreased (all P<0.05), in which sh-CD147+sh-PDGF-CC group was the lowest. The number of migrating cells in sh-CD147 group, sh-PDGF-CC group and sh-CD147+sh-PDGF-CC group was lower than that in Mock group and Ctrl RNA group［（20.7±4.5）,（13.7±2.5）,（7.3±2.1） vs （50.0±4.6）,（51.3±9.5）］(F=45.130, P<0.001), and the number in sh-CD147+sh-PDGF-CC group was lower than that in sh-CD147 group and sh-PDGF-CC group (F=12.910, P=0.006). Conclusions  In TNBC cell line MDA-MB-231, the expression PDGF-CC is higher in high-CD147 expression subcellular line. Down-regulation of both CD147 and PDGF-CC can play a synergistic role in inhibiting cell proliferation and migration.