2021 年第 12 期 第 16 卷

24小时尿标本生化检测中钙磷镁离子测定值的影响因素分析

Influencing factors of calcium, phosphorus and magnesium ions measured value in 24-hour urine specimen biochemical detection

作者：李学武1张晓1王清吟2肖征1

英文作者：Li Xuewu1 Zhang Xiao1 Wang Qingyin2 Xiao Zheng1

单位：1中国人民解放军总医院海南医院检验科，海南省三亚市572000；2浙江省宁波市奉化区人民医院医共体总院输血科315000

英文单位：1Department of Clinical Laboratory Hainan Hospital of Chinese PLA General Hospital Hainan Povince Sanya 572000 China; 2Department of Blood Transfusion General Hospital of Medical Community of Fenghua People′s Hospital Zhejiang Province Ningbo 315000 China

关键词：尿生化检测；离子；24小时尿标本；pH值；放置时长

英文关键词：Urinebiochemistrytest;Ion;24-hoururinespecimen;pHvalue;Storagetime

• 摘要：

• 目的 探讨24 h尿标本生化检测中钙磷镁离子测定值的影响因素。方法 选取2020年3—6月中国人民解放军总医院海南医院门诊或住院患者的61份24 h新鲜尿标本。每份标本分成4份，其中3份按照试剂盒推荐的检测pH值（钙离子3~4、磷离子<3、镁离子1）以盐酸分别酸化尿液，分别测定各份标本常温条件下即时（0 h）及放置24 h后钙磷镁离子的浓度，对各条件下测定值分别进行比较。结果  常温即时检测中酸化后磷镁离子含量低于未酸化时［（11.16±0.86）mmol/24 h比（11.36±0.88）mmol/24 h、（1.82±0.14）mmol/24 h比（1.84±0.14）mmol/24 h］，差异均有统计学意义（均P<0.05）。未酸化标本放置24 h后钙磷离子含量均低于常温即时［钙离子：（1.91±0.21）mmol/24 h比（2.08±0.22）mmol/24 h，放置24 h后磷离子：（11.15±0.88）mmol/24 h］，差异均有统计学意义（均P<0.05）。酸化尿标本放置24 h后磷离子含量［（11.42±0.86）mmol/24 h］高于即时检测，差异有统计学意义（P<0.05）。放置24 h后，酸化尿标本钙离子［（2.14±0.22）mmol/24 h］、磷离子检测结果高于未酸化标本，差异均有统计学意义（均P<0.05）。结论  在24 h尿标本的检测中，标本的pH值及放置时长对于钙磷镁离子的测定值存在不同影响。

• Objective To investigate the influencing factors of calcium, phosphorus and magnesium ions measured value in 24-hour urine specimen biochemical detection. Methods Totally 61 fresh 24 h urine samples from outpatients or inpatients in Hainan Hospital of Chinese PLA General Hospital from March to June 2020 were selected. Each sample was divided into 4 parts, of which 3 parts were acidified with hydrochloric acid according to the detection pH value recommended by the kit(calcium 3-4, phosphorus<3, magnesium 1). The concentrations of calcium, phosphorus and magnesium ions in each part of sample were measured real-time (0 h) at normal temperature and 24 h after storage, and the measured value under each condition was compared. Results In real-time detection at normal temperature, the contents of phosphorus and magnesium ions after acidified were lower than those non acidified［（11.16±0.86）mmol/24 h vs （11.36±0.88）mmol/24 h, （1.82±0.14）mmol/24 h vs （1.84±0.14）mmol/24 h］(all P<0.05). The contents of calcium and phosphorus ions in non acidified samples 24 h after storage were lower than those in real-time at normal temperature［calcium ion: (1.91±0.21)mmol/24 h vs (2.08±0.22)mmol/24 h, phosphorus ion 24 h after storage: (11.15±0.88)mmol/24 h］, and the differences were statistically significant(all P<0.05). The content of phosphorus ion in acidified urine samples 24 h after storage［（11.42±0.86）mmol/24 h］ was higher than that in real-time detection, and the difference was statistically significant(P<0.05). After 24 h of storage, detection results of calcium ［(2.14±0.22)mmol/24 h］ and phosphorus ions in acidified urine samples were higher than those in non acidified samples, and the differences were statistically significant(both P<0.05). Conclusion  In the 24 h urine sample detection, the measured values of calcium, phosphorus and magnesium ions are significantly different influenced by the pH value and storage time.