2021 年第 6 期 第 16 卷

右美托咪定对气腹后脂多糖诱导肝损伤模型小鼠肝功能的保护作用研究

The protective effect of dexmedetomidine on liver function in model mice undergoing lipopolysaccharide-inducing liver injury after pneumoperitoneum

作者：杨毅杨男黎娟娟付莉娟郭祯华贺文伟

英文作者：Yang Yi Yang Nan Li Juanjuan Fu Lijuan Guo Zhenhua He Wenwei

单位：大理大学第一附属医院麻醉科671000

英文单位：Department of Anesthesiology the First Affiliated Hospital of Dali University Yunnan Province Dali 671000 China

关键词：肝损伤；右美托咪定；气腹；炎症；氧化应激

英文关键词：Liverinjury;Dexmedetomidine;Pneumoperitoneum;Inflammation;Oxidativestress

• 摘要：

• 目的 研究右美托咪定(DEX)对气腹后脂多糖诱导肝损伤模型小鼠肝功能的保护作用。方法 将40只无特定病原体级小鼠完全随机分为对照组(不进行处理)、气腹组、气腹+脂多糖处理组及气腹+脂多糖+DEX处理组，各10只。检测比较各组肝功能指标［丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）］、炎症因子指标［白细胞介素（IL）-1、IL-4、IL-6、肿瘤坏死因子α(TNF-α)］、氧化应激指标［活性氧、丙二醛、诱导型一氧化氮合酶（iNOS）、超氧化物歧化酶（SOD）］表达水平。结果 气腹+脂多糖处理组和气腹+脂多糖+DEX处理组的ALT和AST水平均明显高于对照组及气腹组，气腹+脂多糖+DEX处理组的ALT和AST水平均明显低于气腹+脂多糖处理组［（68±5）U/L比（106±9）U/L、（56±5）U/L比（77±8）U/L］，差异均有统计学意义(均P＜0.05)。气腹+脂多糖处理组和气腹+脂多糖+DEX处理组的IL-1、IL-4、 IL-6、TNF-α mRNA水平，活性氧、丙二醛、iNOS mRNA和SOD mRNA水平均明显高于气腹组，气腹+脂多糖+DEX处理组的以上指标水平均明显低于气腹+脂多糖处理组，差异均有统计学意义(均P＜0.05)。结论 DEX可以保护气腹后脂多糖诱导肝损伤模型小鼠的肝功能，减轻炎症反应以及氧化应激。

• Objective To investigate the protective effect of dexmedetomidine(DEX) on liver function in model mice undergoing lipopolysaccharide(LPS)-inducing liver injury after pneumoperitoneum. Methods Totally 40 specific pathogen free mice were randomly divided into the control group (no treatment), the pneumoperitoneum group, the pneumoperitoneum+LPS group and the pneumoperitoneum+LPS+DEX group, with 10 mice in each group. The levels of liver function indexes［alanine aminotransferase(ALT), aspartate aminotransferase(AST)］, inflammatory factors indexes ［interleukin(IL)-1, IL-4, IL-6, tumor necrosis factor-α(TNF-α)］, oxidative stress indexes［reactive oxygen species, malondialdehyde, inducible nitric oxide synthase(iNOS), superoxide dismutase(SOD)］ were detected and compared among the groups. Results ALT and AST levels of the pneumoperitoneum+LPS group and the pneumoperitoneum+LPS+DEX group were significantly higher than those of the control group and the pneumoperitoneum group, and ALT and AST levels of the pneumoperitoneum+LPS+DEX group were significantly lower than those of pneumoperitoneum+LPS group［(68±5)U/L vs (106±9)U/L, (56±5)U/L vs (77±8)U/L］, with significant differences (all P<0.05). The levels of IL-1, IL-4, IL-6, TNF-α mRNA, reactive oxygen species, malondialdehyde, iNOS mRNA and SOD mRNA of the pneumoperitoneum+LPS group and the pneumoperitoneum+LPS+DEX group were significantly higher than those of the pneumoperitoneum group, and those of the pneumoperitoneum+LPS+DEX group were significantly lower than those of the pneumoperitoneum+LPS group(all P<0.05). Conclusion DEX can protect the liver function of model mice with LPS-inducing liver injury after pneumoperitoneum, and reduce inflammatory reaction and oxidative stress.