2021 年第 4 期 第 16 卷

非小细胞肺癌组织长链非编码RNA LINC01503与微小RNA-335-5p的表达及其临床意义

Expressions and clinical significances of long non-coding RNA LINC01503 and microRNA-335-5p in non-small cell lung cancer tissues

作者：蔡亮傅文生谷士海

英文作者：Cai Liang Fu Wensheng Gu Shihai

单位：安徽医科大学附属宿州医院胸心外科234000

英文单位：Department of Cardiothoracic Surgery Suzhou Hospital of Anhui Medical University Suzhou 234000 China

关键词：非小细胞肺癌；长链非编码RNALINC01503；微小RNA-335-5p；TNM分期

英文关键词：Non-smallcelllungcancer;Longnon-codingRNALINC01503;MicroRNA-335-5p;TNMstage

• 摘要：

• 目的 探究非小细胞肺癌（NSCLC）组织中长链非编码RNA（lncRNA）LINC01503、微小RNA（miR）-335-5p的表达及其临床意义。方法 选取2016年1—12月于安徽医科大学附属宿州医院诊治的88例NSCLC患者，采用实时荧光定量聚合酶链反应检测癌组织和癌旁组织中lncRNA LINC01503、miR-335-5p的表达量，分析癌组织中lncRNA LINC01503、miR-335-5p表达的相关性及随访期间患者的生存情况。结果 NSCLC癌组织中lncRNA LINC01503表达量明显高于癌旁组织［（2.63±0.30）比（1.05±0.21）］，miR-335-5p表达量明显低于癌旁组织［（1.14±0.23）比（1.92±0.22）］，差异均有统计学意义（均P<0.001）。TNM Ⅲ期患者癌组织中lncRNA LINC01503表达量高于TNM Ⅰ～Ⅱ期、miR-335-5p表达量低于TNM Ⅰ～Ⅱ期，差异均有统计学意义（均P＜0.001）。癌组织中lncRNA LINC01503与miR-335-5p的表达量呈负相关（r=-0.612，P<0.001）。lncRNA LINC01503高表达患者3年生存率及平均生存时间显著低于/短于lncRNA LINC01503低表达患者，miR-335-5p高表达患者3年生存率及平均生存时间显著高于/长于miR-335-5p低表达患者（均P＜0.05）。结论 NSCLC癌组织中lncRNA LINC01503表达量升高，miR-335-5p表达量下降，二者表达量呈负相关，可能成为提示NSCLC预后新的肿瘤标志物。

• Objective To investigate the expressions and clinical significances of long non-coding RNA (lncRNA) LINC01503 and microRNA (miR)-335-5p in non-small cell lung cancer (NSCLC) tissues. Methods From January to December 2016, 88 cases of NSCLC patients admitted to Suzhou Hospital of Anhui Medical University were selected. Real-time fluorescence quantitative polymerase chain reaction was used to detect the expressions of lncRNA LINC01503 and miR-335-5p in cancer tissues and adjacent tissues. The correlation between the expressions of lncRNA LINC01503 and miR-335-5p in cancer tissues and the survival of patients in follow-up period were analyzed. Results The expression of lncRNA LINC01503 in NSCLC cancer tissues was significantly higher than that in adjacent tissues［（2.63±0.30） vs （1.05±0.21）］, while the expression of miR-335-5p in NSCLC cancer tissues was significantly lower than that in adjacent tissues ［（1.14±0.23） vs （1.92±0.22）］ (both P<0.001). The expression of lncRNA LINC01503 in cancer tissues of patients with TNM stage Ⅲ was higher than that of patients with stage Ⅰ-Ⅱ, and the expression of miR-335-5p in cancer tissues of patients with TNM stage Ⅲ was lower than that of patients with stage Ⅰ-Ⅱ(both P<0.001). There was a negative correlation between the expressions of lncRNA LINC01503 and miR-335-5p in cancer tissues (r=-0.612, P<0.001). The 3-year survival rate and average survival time of patients with high expression of lncRNA LINC01503 were significantly lower/shorter than those of patients with low expression of lncRNA LINC01503, and 3-year survival rate and average survival time of patients with high expression of miR-335-5p were significantly higher/longer than that of patients with low expression of miR-335-5p (all P<0.05). Conclusions  The expression of lncRNA LINC01503 increases in NSCLC cancer tissues, while the expression of miR-335-5p decreases. There is a negative correlation between them. They are expected to become new tumor markers for prognosis of NSCLC.