2021 年第 3 期 第 16 卷

白藜芦醇对卵巢癌细胞顺铂化疗的增敏作用和机制

Sensitization effect and mechanism of resveratrol on cisplatin chemotherapy in ovarian cancer cells

作者：王慧贺英陈丽华王思思王静马春星焦桂青亢延飞

英文作者：Wang Hui He Ying Chen Lihua Wang Sisi Wang Jing Ma Chunxing Jiao Guiqing Kang Yanfei

单位：河北北方学院附属第一医院妇科,河北省张家口市075000

英文单位：Department of Gynecology the First Affiliated Hospital of Hebei North University Hebei Province Zhangjiakou 075000 China

关键词：卵巢癌；白藜芦醇；顺铂；增敏作用；凋亡

英文关键词：Ovariancancer;Resveratrol;Cisplatin;Sensitization;Apoptosis

• 摘要：

• 目的 探讨白藜芦醇对卵巢癌细胞顺铂化疗的增敏作用和机制。方法 选取人卵巢癌SKOV3顺铂耐药细胞（SKOV3/DDP）分为对照组和观察组。对照组常规培养,不进行药物干预；观察组根据药物干预方式分为顺铂组［顺铂（32 μg/ml）干预24 h］和白藜芦醇+顺铂组［白藜芦醇（20 μg/ml）+顺铂（32 μg/ml）干预24 h］。通过倒置显微镜、细胞计数试剂盒8、流式细胞仪和蛋白质印迹法分别检测3组 SKOV3/DDP的生长状态、细胞抑制率、细胞凋亡情况以及B细胞淋巴瘤2家族蛋白及其相关X蛋白（Bax）和抑癌蛋白p21（p21）的表达情况。结果 对照组SKOV3/DDP呈长梭形，细胞状态较好；顺铂组SKOV3/DDP出现较多圆球形的悬浮细胞，但仍有大部分细胞状态正常；白藜芦醇+顺铂组SKOV3/DDP几乎全部收缩变圆。白藜芦醇+顺铂组、顺铂组细胞抑制率、细胞凋亡率、Bax及p21表达水平均高于对照组，且白藜芦醇+顺铂组均高于顺铂组［（55±4）%比（41±4）%、（41.52±3.24）%比（30.21±1.21）%、（6.2±0.7）比（4.1±0.4）、（3.69±0.39）比（2.56±0.61）］，差异均有统计学意义（均P<0.05）。结论 白藜芦醇可增加SKOV3/DDP对顺铂化疗敏感性，促进SKOV3/DDP细胞内Bax和p21的表达，并促进细胞凋亡。

• Objective To investigate the sensitization effect and mechanism of resveratrol (Res) on cisplatin （DDP） chemotherapy in ovarian cancer cells. Methods The DDP-resistant human ovarian cancer cell SKOV3 (SKOV3/DDP) were divided into control group and observation group. The control group was cultured conventionally without drug intervention. The observation group was divided into DDP group［intervened by DDP（32 μg/ml） for 24 h］ and Res+DDP group［intervened by Res（20 μg/ml）+DDP（32 μg/ml） for 24 h］. Inverted microscope, cell counting kit-8, flow cytometer and western blotting were used to detect the growth status, cell inhibition rate, apoptosis statusand, and expressions of B cell lumphoma 2（Bcl-2） family protein and Bcl-2-associated X protein (Bax) and tumor suppressor protein 21(p21) of SKOV3/DDP in the three groups. Results SKOV3/DDP in control group were in long spindle shape with good cell state; SKOV3/DDP in DDP group showed more spherical suspension cells, but most of them in normal condition; SKOV3/DDP in Res+DDP group were almost completely contracted and rounded. The cell inhibition rate, apoptosis rate and expression levels of Bax and p21 in Res+DDP group and DDP group were higher than those in control group, and those in Res+DDP group were higher than those in DDP group［（55±4）% vs （41±4）%,（41.52±3.24）% vs （30.21±1.21）%,（6.2±0.7） vs （4.1±0.4）,（3.69±0.39） vs （2.56±0.61）］（all P<0.05）. Conclusion Res can increase the chemotherapy sensitivity of SKOV3/DDP to DDP, promote the expressions of Bax， p21 and accelerate apoptosis.