2020 年第 12 期 第 15 卷

高血压脑出血患者发病早期外周血γ干扰素和T细胞免疫球蛋白黏蛋白域分子3 mRNA的表达及意义研究

The expression and significance of peripheral blood interferon-γ and T cell immunoglobulin mucin-domain containing molecule-3 mRNA in early stage of hypertensive cerebral hemorrhage

作者：肖文峰蒋正方袁淼叶亮朱黎

英文作者：Xiao Wenfeng Jiang Zhengfang Yuan Miao Ye Liang Zhu Li

单位：四川绵阳四〇四医院神经外科621000

英文单位：Department of Neurosurgery Sichuan Mianyang 404 Hospital Mianyang 621000 China

关键词：高血压脑出血；γ干扰素；T细胞免疫球蛋白黏蛋白域分子3；白细胞介素

英文关键词：Hypertensivecerebralhemorrhage;Interferon-γ;Tcellimmunoglobulinmucin-domaincontainingmolecule-3;Interleukin

• 摘要：

• 目的 研究高血压脑出血患者发病早期外周血γ干扰素及外周血单个核细胞中T细胞免疫球蛋白黏蛋白域分子3（TIM-3）mRNA的表达及意义。方法 选择四川绵阳四〇四医院2019年1—12月收治的43例发病至入院时间在6 h内的高血压脑出血患者作为脑出血组；选取同期年龄、性别匹配的健康体检者40名作为对照组。比较对照组与脑出血组发病＜6 h和发病24、48、72 h血清γ干扰素、TIM-3 mRNA表达水平及白细胞介素（IL）-2、IL-4、IL-10水平。结果 脑出血组发病＜6 h和发病24、48、72 h血清γ干扰素、TIM-3 mRNA表达水平均高于对照组［（177±21）、（194±23）、（239±25）、（191±22）ng/L比（125±17）ng/L，（0.208±0.077）、（0.504±0.120）、（0.824±0.148）、（0.561±0.073）比（0.065±0.018）］，差异均有统计学意义（均P＜0.05）。脑出血组发病＜6 h和发病24、48 h血清γ干扰素、TIM-3 mRNA表达水平呈上升趋势，发病72 h时二者表达水平下降，且均低于发病48 h，差异均有统计学意义（均P＜0.05）。脑出血组发病＜6 h和发病24、48、72 h血清IL-2、IL-4、IL-10水平与对照组比较差异均无统计学意义(均P＞0.05)。脑出血组发病不同时间IL-2、IL-4、IL-10水平差异也均无统计学意义(均P＞0.05)。结论 高血压脑出血患者发病早期血清γ干扰素、TIM-3 mRNA表达增加，可能参与脑出血后的病理过程。

• Objective To study the expression and significance of peripheral blood interferon-γ and T cell immunoglobulin mucin-domain containing molecule-3(TIM-3) mRNA in early stage of hypertensive cerebral hemorrhage. Methods From January 2019 to December 2019， 43 patients with hypertensive cerebral hemorrhage within 6 h from onset to admission in Sichuan Mianyang 404 Hospital were selected as the cerebral hemorrhage group. Forty healthy physical examination people in the same period were selected as the control group. Serum levels of interferon, TIM-3 mRNA and interleukin (IL)-2, IL-4 and IL-10 were compared between the control group and the cerebral hemorrhage group at < 6 h and 24, 48 and 72 h after onset respectively. Results The expressions of interferon-γ and TIM-3 mRNA in the cerebral hemorrhage group at＜6 h, 24 h, 48 h and 72 h after onset were higher than those in the control group［（177±21）, （194±23）, （239±25）, （191±22）ng/L vs （125±17）ng/L; （0.208±0.077）, （0.504±0.120）, （0.824±0.148）, （0.561±0.073） vs （0.065±0.018）， all P＜0.05］. The expression levels of interferon-γ and TIM-3 mRNA in the cerebral hemorrhage group increased at ＜6 h, 24 and 48 h after onset, and decreased at 72 h after onset, which were lower than those at 48 h after onset (all P<0.05). Serum levels of IL-2, IL-4, and IL-10 in the cerebral hemorrhage group at <6, 24, 48, 72 h after onset were not statistically different from those in the control group (all P>0.05). There were no significant differences in the levels of IL-2, IL-4, and IL-10 in the cerebral hemorrhage group at different times of onset (all P>0.05). Conclusion The expression of interferon-γ and TIM-3 mRNA in patients with hypertensive intracerebral hemorrhage increased in the early stage of onset, which may be involved in the pathological process after cerebral hemorrhage.