2020 年第 9 期 第 15 卷

山东地区人群中EB病毒感染情况的血清流行病学分析

Epidemiological study of Epstein-Barr virus infection in Shandong province

作者：张庆刘义庆李丽刘春梅邱旸鞠瑛张炳昌

英文作者：Zhang Qing Liu Yiqing Li Li Liu Chunmei Qiu Yang Ju Ying Zhang Bingchang

单位：山东大学附属省立医院临床医学检验部,济南250021

英文单位：Department of Clinical Laboratory Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021 China

关键词：EB病毒；抗体；化学发光免疫分析；血清流行病学

英文关键词：Epstein-Barrvirus；Antibody；Chemiluminescenceimmunoassay；Seroepidemiology

• 摘要：

• 目的 了解山东地区人群中EB病毒感染的血清流行病学情况。方法 选取2015年9月至2018年8月在山东大学附属省立医院就诊的EB病毒疑似感染者9 445例。用化学发光免疫分析方法检测患者血清中抗EB病毒衣壳抗原抗体免疫球蛋白（Ig）G（VCA IgG）、抗EB病毒衣壳抗原抗体IgM（VCA IgM）、抗EB病毒早期抗原抗体IgG（EA-D IgG）和抗EB病毒核抗原抗体IgG（EBNA IgG）情况。结果 9 445例患者中VCA IgG阳性7 821例（82.81%），VCA IgM阳性935例（9.90%），EA-D IgG阳性513例（5.43%），EBNA IgG阳性7 311例（77.41%）。男性患者VCA IgG、EA-D IgG、EBNA IgG阳性率均低于女性，差异均有统计学意义（均P＜0.05）。不同年龄患者间VCA IgG、VCA IgM、EA-D IgG、EBNA IgG阳性率差异均有统计学意义（均P＜0.001）。VCA IgM阳性率冬季最高为12.58%（279/2 217），秋季为11.45%（247/2 158），夏季为8.56%（230/2 686），春季最低为7.51%（179/2 384），季节之间差异有统计学意义（P＜0.05）。共检出16种EB病毒抗体组合模式，其中以VCA IgG、EBNA IgG双阳性模式最多见，共检出6 235例，占66.01%。结论 山东地区人群EB病毒抗体阳性率存在性别、年龄和季节差异，EB病毒抗体组合感染模式以VCA IgG和EBNA IgG双阳性的既往感染模式为主，其他多种感染模式并存。

• Objective To investigate the prevalence of Epstein-Barr (EB) virus infection in the population of Shandong. Methods  From September 2015 to August 2018, 9 445  suspected EB virus patients admitted to Shandong Provincial Hospital Affiliated to Shandong University were collected. Serum EB virus capsid antigen antibody(VCA) immunoglobulin(Ig)G(VCA IgG), VCA-IgM, EB virus early antigen-D antibody IgG(EA-D IgG) and EB virus nuclear antigen antibody IgG(EBNA IgG) were detected by chemiluminescence immunoassay. Results In 9 445 cases, the positive rates of VCA IgG, VCA IgM, EA-D IgG and EBNA IgG were 82.81%(7 821/9 445), 9.90%(935/9 445), 5.43%(513/9 445) and 77.41%(7 311/9 445), respectively. The positive rates of VCA IgG, EA-D IgG and EBNA IgG in male patients were significantly lower than those in the female patients (all P＜0.05). There were significant differences in the positive rates of VCA IgG, VCA IgM, EA-D IgG and EBNA IgG among patients of different ages (all P＜0.001). The positive rate of VCA IgM was 12.58%(279/2 217) in winter, 11.45%(247/2 158) in autumn and 8.56%(230/2 686) in summer, but 7.51%(179/2 384) in spring(P＜0.05). A total of 16 combinations of EB virus antibody combinations were detected, VCA IgG and EBNA IgG double positive pattern was the most common, a total of 6 235 cases（66.01%） were detected. Conclusions The positive rate of EB virus antibodies in Shandong shows differences in gender, age and season. The predominant infection patterns of VCA IgG and EBNA IgG double positive are complicated.