2020 年第 9 期 第 15 卷

人参皂苷Rb1对心力衰竭大鼠线粒体膜电位的影响 

Effect of ginsenoside Rbl on mitochondrial membrane potential in rats with heart failure 

作者：孔宏亮1赵雨婷2蒋玉昆1田美景2王惠盼2马草滨1

英文作者：Kong Hongliang1 Zhao Yuting2 Jiang Yukun1 Tian Meijing2 Wang Huipan2 Ma Caobin1

单位：1辽宁省人民医院中国医科大学人民医院心脏中心，沈阳110013；2大连医科大学在读硕士研究生116044 

英文单位：1Cardiology Center the People′s Hospital of Liaoning Province the People′s Hospital of China Medical University Shenyang 110013 China; 2Postgraduate of Dalian Medical University Dalian 116044 China

关键词：心力衰竭；人参皂苷Rb1；线粒体膜电位；电压依赖性阴离子通道

英文关键词：Heartfailure;Ginsenosides-Rb1;Mitochondrialmembranepotential;Voltagedependentanionchannel

• 摘要：

• 目的 探讨人参皂苷Rb1（Gs-Rb1）对心力衰竭大鼠线粒体膜电位的影响。方法阿霉素诱导的心力衰竭大鼠随机分为心力衰竭组(16只)和Gs-Rb1组(18只)，另外10只大鼠按阿霉素注射方法注射等量0.9%氯化钠注射液作为对照组。干预4周后，均完成心脏超声左心室射血分数(LVEF)检查。分别测定大鼠血清N末端B型脑钠肽前体（NT-proBNP）水平、线粒体膜电位和线粒体肿胀度，同步蛋白质印迹法检测线粒体钠钙交换体(NCLX)、线粒体钙离子单向转运体(MCU)、电压依赖性阴离子通道(VDAC)和线粒体钙离子摄入蛋白1（MICU1）等蛋白水平。结果 Gs-Rb1组LVEF高于心力衰竭组，而NT-proBNP水平低于心力衰竭组［（47±3）%比（37±5）%、（774±200）ng/L比（2 073±827）ng/L］(均P<0.01)。Gs-Rb1组线粒体膜电位高于心力衰竭组，线粒体肿胀度小于心力衰竭组（均P<0.01）。Gs-Rb1组NCLX、VDAC、MICU1水平显著高于心力衰竭组，MCU水平低于心力衰竭组(均P<0.01)。结论 Gs-Rb1可能通过调节心肌细胞内线粒体膜电位改善心力衰竭，其可能通过NCLX等实现。

• Objective To discuss the effect of ginsenoside Rbl(Gs-Rb1) on mitochondrial membrane potential in rats with heart failure(HF). Methods Adriamycin-induced rats with HF were randomly divided into HF group(16 rats) and Gs-Rb1 group(18 rats);0.9% sodium chloride injection group was as control(10 rats). After 4 weeks of intervention, the left ventricular ejection fraction(LVEF) was analyzed by echoeardiography. Serum lipid N-terminal B-type natriuretic peptide(NT-proBNP), mitochondrial membrane potential and mitochondrial swelling were anlyzed. Mitochondrial Na+/Ca2+ exchanger(NCLX), mitochondrial calcium uniporter(MCU), voltage dependent anion channel(VDAC) and mitochondrial calcium uptake 1(MICU1) were analyzed by western bolt. Results The LVEF in Gs-Rb1 group was higher than that in HF group, the NT-proBNP level was lower than that in HF group［（47±3）% vs （37±5）%,（774±200）ng/L vs （2 073±827）ng/L］(both P＜0.01). The mitochondrial membrane potential in Gs-Rb1 group was higher than that in HF group; the mitochondrial swelling was lower than that in HF group (both P＜0.01). The levels of NCLX, VDAC and MICU1 in Gs-Rb1 group were significantly higher than those in HF group, and the level of MCU in Gs-Rb1 group was lower than that in HF group (all P＜0.01). Conclusion Gs-Rb1 may improve HF by regulating mitochondrial membrane potential in cardiomyocytes, which may be related to NCLX.