2019 年第 8 期 第 14 卷

基质细胞衍生因子1和 CXC类趋化因子受体7对β抑制蛋白调控肺癌细胞转移与侵袭作用的影响

Effects of stromal cell-derived factor-1 and CXC chemokine receptor-7 on β-arrestin regulating metastasis and invasion of lung cancer cells

作者：苟志斌朱建勇刘海娟张立波何敏叶青范荣梅李俊敏郭光云

英文作者：

单位：442000湖北省十堰市人民医院湖北医药学院附属人民医院呼吸内科I病区(苟志斌、朱建勇、张立波、何敏、叶青、范荣梅、李俊敏、郭光云)，检验科（刘海娟）

英文单位：

关键词：肺癌；基质细胞衍生因子1；CXC类趋化因子受体7；β抑制蛋白

英文关键词：

• 摘要：

• 【摘要】目的    探讨基质细胞衍生因子1（SDF-1）和CXC类趋化因子受体7（CXCR7）对β抑制蛋白调控肺癌细胞转移与侵袭作用的影响。方法    取对数生长期肺癌A549细胞系，分别转染CXCR7(CXCR7转染组)、空载质粒(转染对照组)，同时设置不进行转染的空白对照组，采用噻唑蓝法检测细胞增殖抑制情况，流式细胞术检测细胞凋亡情况，Transwell实验检测细胞转移与侵袭情况，实时荧光定量聚合酶链反应法检测SDF-1和CXCR7 mRNA的表达，蛋白质印迹法检测蛋白表达情况。结果    转染后48、72 h，CXCR7转染组SDF-1和CXCR7 mRNA相对表达量低于转染对照组和空白对照组(均P<0.05)，细胞增殖抑制率、细胞凋亡率均低于转染对照组和空白对照组［转染48 h：(3.2±1.3)%比（10.4±3.3）%、（11.9±3.3）%，(4.4±1.8)%比（16.8±2.2）%、（18.9±3.2）%；转染72 h：(2.1±1.1)%比（14.0±2.1）%、（13.8±1.8）%，(5.4±1.2)%比（18.3±3.7）%、（20.1±2.8）%］(均P<0.05)。转染后48 h，CXCR7转染组的细胞转移与侵袭比例以及SDF-1、CXCR7、β抑制蛋白相对表达量均高于转染对照组和空白对照组(均P<0.05)。结论    SDF-1/CXCR7能促进β抑制蛋白的表达，从而促进肺癌细胞增殖、转移与侵袭，抑制细胞凋亡。

• 【Abstract】Objective    To explore the effects of stromal cell-derived factor-1(SDF-1) and CXC chemokine receptor-7(CXCR7) on metastasis and invasion of lung cancer cells regulated by β-arrestin. Methods    Lung cancer cell line A549 were transfected by CXCR7(CXCR7 group) and empty plasmid（transfection control group）; A549 cells with no treatment were blank control group. Cell proliferation was detected by MTT assay; apoptosis was detected by flow cytometry; metastasis and invasion were detected by Transwell assay; SDF-1 and CXCR7 mRNA expression was detected by real-time quantitative polymerase chain reaction; protein expression was detected by western blotting. Results    At 48 and 72 h after transfection, relative expression levels of SDF-1 and CXCR7 mRNA in CXCR7 group were significantly lower than those in transfection control group and blank control group(all P<0.05); cell proliferation inhibition rate and apoptosis rate in CXCR7 group were significantly lower than those in transfection control group and blank control group［48 h: (3.2±1.3)% vs （10.4±3.3）%,（11.9±3.3）%; (4.4±1.8)% vs （16.8±2.2）%,（18.9±3.2）%; 72 h : (2.1±1.1)% vs （14.0±2.1）%,（13.8±1.8）%; (5.4±1.2)% vs （18.3±3.7）%,（20.1±2.8）%］(all P<0.05). At 48 h after transfection, cell metastasis rate and invasion rate, relative expression levels of SDF-1, CXCR7 and β-arrestin in CXCR7 group were significantly higher than those in transfection control group and blank control group(all P<0.05). Conclusion    SDF-1/CXCR7 can increase β-arrestin expression and promote proliferation, metastasis and invasion, inhibit apoptosis of lung cancer cells.