2019 年第 3 期 第 14 卷

小分子RNA_Cdc42_GTPase和微小RNA-363-5p在不同级别胶质细胞瘤中的分布规律

Distribution of small RNA Cdc42 GTPase and microRNA-363-5p in different grades of glioma

作者：周浪浪陈胜利

英文作者：

单位：030012太原，山西医科大学附属人民医院山西省人民医院神经外科（周浪浪为山西医科大学2016级神经外科专业在读硕士研究生）

英文单位：

关键词：神经胶质细胞瘤；Cdc42GTPase；微小RNA-363-5p；细胞增殖；细胞凋亡

英文关键词：

• 摘要：

• 【摘要】目的    探讨Cdc42 GTPase和微小RNA（miRNA）-363-5p在不同级别胶质细胞瘤中的分布规律。方法    常规采集胶质细胞瘤患者术后肿瘤样本，多聚甲醛固定24 h后，用蔗糖溶液浸泡至沉底，冰冻切片后应用免疫组织化学方法检测Cdc42 GTPase的表达；其余样品用液氮保存，实时荧光定量-聚合酶链反应检测Cdc42 GTPase和miRNA-363-5p在不同级别胶质细胞瘤中的表达情况。结果    实时荧光定量-聚合酶链反应检测结果显示，随着肿瘤级别（肿瘤临床分期Ⅰ、Ⅱ、Ⅲ、Ⅳ期）的增高，miRNA-363-5p表达量逐渐上调［（1.401±0.515）×10-6、（1.665±0.686）×10-6、（5.792±0.000）×10-6、（8.383±2.858）×10-6］，而Cdc42 GTPase 表达量逐渐下调［(2.218±0.694)×10-2、（2.004±0.236）×10-2、（0.285±0.004）×10-2、（0.071±0.007）×10-2］，两两比较差异均有统计学意义（均P<0.05）。结论    miRNA-363-5p的靶基因可能是Cdc42 GTPase，且随着miRNA-363-5p表达量的上调，Cdc42 GTPase表达量下调，胶质细胞瘤级别增高。

• 【Abstract】Objective    To investigate the distribution of small RNA Cdc42 GTPase and microRNA（miRNA）-363-5p in different grades of glioma. Methods    Glioma samples of human were fixed by paraformaldehyde for 24 h and immersed in sucrose solution until it sank to the bottom. Partial tissues were made frozen section to test the expression of Cdc42 GTPase by immunohistochemistry. The rest samples were stored in liquid nitrogen, quantitative real-time polymerase chain reaction was used to detect expressions of Cdc42 GTPase and miRNA-363-5p in different grades of tumor. Results    Polymerase chain reaction showed that the expression of miRNA-363-5p gradually increased［（1.401±0.515）×10-6, （1.665±0.686）×10-6, （5.792±0.000）×10-6, （8.383±2.858）×10-6］ and the expression of Cdc42 GTPase was down-regulated［(2.218±0.694）×10-2, （2.004±0.236）×10-2, （0.285±0.004）×10-2,（0.071±0.007）×10-2］ with the increase of tumor grade; there were significant differences in multiple comparisons(all P<0.05). Conclusion    Cdc42 GTPase may be the target gene of miRNA-363-5p and they changed in an opposite direction of trend; up-regulation of miRNA-363-5p and down-regulation of Cdc42 GTPase indicate high grade of glioma.