2018 年第 12 期 第 13 卷

局部给予5-羟色胺2受体激动剂或拮抗剂对SD大鼠舌下神经核兴奋性的影响

Effects of local use of 5-hydroxytryptamine-2 receptor agonist and antagonist on excitability of hypoglossal nucleus in SD rats

作者：王伟王广发闫春良薛旗山杨国辉胡系伟

英文作者：

单位：100076北京航天总医院呼吸内科（王伟、闫春良、薛旗山）；100034北京大学第一医院呼吸内科（王广发）；550004贵阳，贵州医科大学附属医院重症医学科（杨国辉），呼吸内科（胡系伟）

英文单位：

关键词：舌下神经核；SD大鼠；5-羟色胺2受体

英文关键词：

• 摘要：

• 【摘要】目的    观察5-羟色胺2(5-HT2)受体激动剂及5-HT2受体拮抗剂对SD大鼠舌下神经核兴奋性的影响。方法    将16只成年雄性SD大鼠完全随机分为A、B组，各8只，均置入微透析导管于其舌下神经核。A组通过微透析导管向舌下神经核泵入新鲜人工脑脊液（ACSF）、新鲜5-HT2受体激动剂溶液，B组通过微透析导管向舌下神经核泵入新鲜ACSF、新鲜5-HT2受体拮抗剂溶液，监测A组未给药时、泵入ACSF时、泵入5-HT2受体激动剂溶液时和B组未给药时、泵入ACSF时、泵入5-HT2受体拮抗剂溶液时的颏舌肌肌电，利用区间测量方法计算颏舌肌肌电面积。结果    A组未给药时、泵入ACSF时、泵入5-HT2受体激动剂时大鼠颏舌肌肌电面积分别为（0.80±0.11）、（0.82±0.12）、（0.93±0.14）mV·s，未给药时与泵入ACSF时肌电面积比较，差异无统计学意义（P=0.280），泵入5-HT2受体激动剂时肌电面积明显大于未给药时与泵入ACSF时，差异均有统计学意义（P=0.005、0.005）。B组未给药时、泵入ACSF时、泵入5-HT2受体拮抗剂时大鼠颏舌肌肌电面积分别为（1.05±0.18）、（1.04±0.19）、（0.73±0.13）mV·s，未给药时与泵入ACSF时肌电面积比较，差异无统计学意义（P=0.955），泵入5-HT2受体拮抗剂时肌电面积明显小于未给药时与泵入ACSF时，差异均有统计学意义（P=0.003、0.003）。结论    5-HT2受体激动剂可以提高舌下神经核的兴奋性，5-HT2受体拮抗剂可以降低舌下神经核的兴奋性。

• 【Abstract】Objective    To observe the effects of 5-hydroxytryptamine-2(5-HT2) receptor agonist and antagonist on hypoglossal nerve nuclear excitability in SD rats. Methods    Sixteen adult male SD rats were randomly divided into group A and group B, with 8 rats in each group. The group A was injected fresh artificial cerebrospinal fluid（ACSF） and 5-HT2 receptor agonist, and the group B was injected ACSF and 5-HT2 receptor antagonist through microdialysis catheter into hypoglossal nucleus. Genioglossus electromyography was monitored before drug use, after giving ACSF and 5-HT2 agonist/antagonist. Results    In the group A, genioglossus electromyography areas before drug use［（0.80±0.11）mV·s］ and after giving ACSF［（0.82±0.12）mV·s］ were similar（P=0.280）; the electromyography area after giving 5-HT2 agonist［（0.93±0.14）mV·s］ was significantly greater than that before drug use and after using ACSF（P=0.005, 0.005）. In the group B, genioglossus electromyography areas before drug use［（1.05±0.18）mV·s］ and after using ACSF［（1.04±0.19）mV·s］ were similar（P=0.955）; the electromyography area after giving 5-HT2 antagonist［（0.73±0.13）mV·s］ was significantly smaller than that before drug use and after giving ACSF（P=0.003, 0.003）. Conclusion    5-HT2 receptor agonist can improve the excitability of hypoglossal nucleus; 5-HT2 receptor antagonist can inhibit the excitability of hypoglossal nucleus.