2018 年第 11 期 第 13 卷

十二井穴针刺对脊髓损伤大鼠脊髓功能恢复及转化生长因子β/Smad通路的影响

Effect of Twelve Jing acupuncture on spinal function recovery and transforming growth factor-β/Smad pathway in rats with spinal cord injury

作者：王恺张赛陈旭义张景童朱仕辉孙明林刘洋

英文作者：

单位：300162天津，武装警察部队后勤学院附属医院脊柱科［王恺（现工作单位为91428部队卫生队、武装警察部队后勤学院二大队）、孙明林］,脑科中心（张赛、陈旭义）；300162天津，武装警察部队后勤学院二大队（张景童、朱仕辉）；200081上海市第四人民医院神经内科（刘洋）

英文单位：

关键词：脊髓损伤；十二井穴；脊髓水肿；转化生长因子β

英文关键词：

• 摘要：

• 【摘要】目的    探讨十二井穴针刺对脊髓损伤大鼠脊髓功能恢复及转化生长因子β(TGF-β)/Smad通路的影响。方法    将40只雌性SD大鼠随机分为5组，即对照组、轻度脊髓损伤组、重度脊髓损伤组、轻度脊髓损伤+井穴针刺组和重度脊髓损伤+井穴针刺组，每组8只。应用动脉瘤夹损伤法构建轻、重度脊髓损伤大鼠模型；联合针刺的2组以1 ml注射器针头点刺大鼠双侧前肢趾尖十二井穴位点，2次/d，每次20 min。2周后，行脊髓损伤评分（BBB评分）和斜坡实验，随后取损伤部位长约3 mm脊髓组织测含水量，另取10 mg损伤周边组织，检测TGF-β、Smad2和P21的基因表达。结果    各脊髓损伤组大鼠与对照组比较，BBB评分和斜坡实验角度明显低/小，脊髓组织含水量明显高，TGF-β、Smad2表达明显高，P21基因表达明显低，差异均有统计学意义（均P<0.05）。轻度脊髓损伤+井穴针刺组与轻度脊髓损伤组比较，BBB评分和斜坡实验角度明显高/大，脊髓组织含水量明显低，TGF-β和Smad2基因表达明显低，P21基因表达明显高［（2.0±0.8）分比（1.2±0.5）分、（32.5±2.7）°比（28.1±2.6）°、（77.3±0.4）%比（77.9±0.5）%、（1.8±0.4）比（2.1±0.6）、（2.59±0.57）比（3.64±0.70）、（0.83±0.12）比（0.69±0.01）］，差异均有统计学意义（均P<0.05）；重度脊髓损伤+井穴针刺组与重度脊髓损伤组比较，各项指标差异均无统计学意义（均P>0.05）。结论    十二井穴针刺可能通过激活TGF-β/Smad相关通路，减少脊髓组织水肿，从而在轻度脊髓损伤中发挥保护作用，但在重度脊髓损伤中单纯应用井穴针刺疗法效果并不显著，应与其他疗法联合应用。

• 【Abstract】Objective    To explore the effect of Twelve Jing acupuncture on spinal function recovery and transforming growth factor(TGF)-β/Smad pathway in rats with spinal cord injury（SCI）. Methods    Forty female SD rats were randomly divided into 5 groups(n=8), including control group, mild SCI group, severe SCI group, mild SCI plus acupuncture group and severe SCI plus acupuncture group. SCI model was made by aneurysm clamp method. Twelve Jing acupoints at bilateral forelimb toes of the rats were acupunctured 2 times/d using needles in the two acupuncture groups, every time 20 min. After 2 weeks, spinal cord injury score(BBB score) assessment and slope test were performed; 3 mm spinal cord tissue at the injured site was taken for water content measurement; 10 mg spinal cord tissue surrounding the lesion was taken for measuring the expressions of TGF-β, Smad2 and P21. Results    BBB score and slope test angle in the mild SCI group and severe SCI group were lower/less, water content of spinal cord tissue was higher, expressions of TGF-β and Smad2 were higher, expression of P21 was lower than those in the control group(all P<0.05). In the mild SCI plus acupuncture group, BBB score and slope test angle were higher/larger, water content of spinal cord tissue was lower, expressions of TGF-β and Smad2 were lower, expression of P21 was higher than those in the mild SCI group［（2.0±0.8） vs （1.2±0.5）, （32.5±2.7）° vs （28.1±2.6）°, （77.3±0.4）% vs （77.9±0.5）%, （1.8±0.4） vs （2.1±0.6）, （2.59±0.57） vs （3.64±0.70）, （0.83±0.12） vs （0.69±0.01）］(all P<0.05). There was no significant difference of these indicators between the severe SCI plus acupuncture group and severe SCI group(P>0.05). Conclusion    sTwelve Jing acupuncture has a therapeutic effect on mild SCI through activating TGF-β/Smad pathways and alleviating edema of spinal cord tissue. In severe SCI, the effect of Twelve Jing acupuncture is not significant.