2018 年第 8 期 第 13 卷

人前梯度蛋白2在垂体腺瘤中的表达及其与细胞内增殖抗原Ki-67和基质金属蛋白酶9及血管内皮生长因子的相关性

Expression of human anterior gradient protein-2 in pituitary adenoma and its association with intracellular proliferative antigen Ki-67, matrix metalloproteinase-9 and vascular endothelial growth factor

作者：麦麦提依明·托合提帕热哈提江·依孜木阿布都克尤木·阿布都吉力力董军吴永刚杨小朋马驰原

英文作者：

单位：830001乌鲁木齐，新疆维吾尔自治区人民医院神经外科（麦麦提依明·托合提、帕热哈提江·依孜木、阿布都克尤木·阿布都吉力力、董军、吴永刚、杨小朋）；210002南京大学医学院附属金陵医院解放军南京总医院神经外科（马驰原）

英文单位：

关键词：垂体腺瘤；人前梯度蛋白2；Ki-67；基质金属蛋白酶9；血管内皮生长因子

英文关键词：

• 摘要：

• 【摘要】目的    研究人前梯度蛋白2(AGR2)在垂体腺瘤组织中的表达及其与细胞内增殖抗原Ki-67、基质金属蛋白酶9(MMP-9)、血管内皮生长因子(VEGF)的相关性。方法    收集2013年5月至2015年 7月在新疆维吾尔自治区人民医院神经外科经手术切除的垂体腺瘤新鲜标本117例。行免疫组织化学染色观察AGR2在垂体腺瘤细胞中的表达，采用Pearson相关分析AGR2表达与Ki67、MMP-9、VEGF的相关性。结果    AGR2在垂体腺瘤不同分泌类型中的表达阳性率差异无统计学意义（χ2=6.537, P>0.05）。在117例垂体腺瘤组织标本中，AGR2表达阳性60例，AGR2在非侵袭性垂体腺瘤组织中的表达明显高于在侵袭性垂体腺瘤中的表达［30.8%（36/47）比20.5%（24/70）］，差异有统计学意义(P<0.01)；AGR2表达与Ki-67、MMP-9、VEGF表达呈负相关（r=-0.252、-0.883、-0.921，均P<0.05）。结论    AGR2在侵袭性垂体腺瘤和非侵袭性垂体腺瘤组织中含量有明显差异。AGR2表达量与Ki-67、MMP-9、VEGF表达量呈负相关，AGR2不仅可以作为研究垂体腺瘤侵袭性的一个靶标，还可以作为垂体腺瘤诊断、靶向治疗以及预后判断的一个重要的潜在指标。

• Objective    To observe the expression of human anterior gradient protein-2(AGR2) in pituitary adenoma and to analyze the relation among AGR2, Ki-67, matrix metalloproteinase-9(MMP-9) and vascular endothelial growth factor(VEGF). Methods    A total of 117 fresh samples of pituitary adenoma were collected from patients who had neurosurgical operation from May 2013 to June 2015 in People′s Hospital of Xinjiang Uygur Autonomous Region. Expression of AGR2 in pituitary adenoma was examined by immunohistochemical staining. Relation among expressions of AGR2, Ki67, MMP-9 and VEGF was analyzed by Pearson test. Results    There was no significant difference of AGR2 positive expression rate among different types of pituitary adenoma（χ2=6.537, P>0.05）. Sixty patients showed positive expression of AGR2; expression rate of AGR2 in noninvasive pituitary adenoma was significantly higher than that in invasive pituitary adenoma［30.8%（36/47） vs 20.5%（24/70）］(P<0.01). Expression of AGR2 in pituitary adenoma was negatively related to expressions of Ki-67, MMP-9 and VEGF（r=-0.252, -0.883, -0.921; P<0.05）. Conclusions    AGR2 expression shows significant difference between noninvasive and invasive pituitary adenoma. AGR2 expression is negatively related to Ki-67, MMP-9 and VEGF expression. AGR2 may be an indicator for diagnosis, treatment and prognosis of pituitary adenoma.