2018 年第 6 期 第 13 卷

免疫抑制剂FTY720与吉西他滨对非小细胞肺癌细胞株癌基因mdig表达的影响

Effects of FTY720 and gemcitabine on expression of oncogene mdig in non-small cell lung cancer cells

作者：李方治侯春阳

英文作者：

单位：110032沈阳，中国医科大学附属第四医院重症医学科（李方治）；110006沈阳市第六人民医院感染科（侯春阳）

英文单位：

关键词：非小细胞肺癌；FTY720；吉西他滨；Mdig基因

英文关键词：

• 摘要：

• 【摘要】目的    分析免疫抑制剂FTY720与吉西他滨对非小细胞肺癌细胞株癌基因mdig表达的影响。方法    将非小细胞肺癌A549及H520细胞株经FTY720、吉西他滨单独处理及联合处理，检测细胞增殖、细胞凋亡及mdig表达情况。结果    采用FTY720对A549及H520细胞处理后可有效抑制细胞增殖，且呈现明显的剂量和时间依赖性。联合组A549及H520细胞的存活率明显低于FTY720组及吉西他滨组［（21.8±1.0）%比（60.1±7.3）%、（44.4±5.0）%，（23.3±1.9）%比（43.6±2.4）%、（65.5±2.9）%］，差异均有统计学意义（均P<0.05）。FTY720联合吉西他滨对A549及H520细胞的药物联合指数均低于1。联合组A549及H520细胞凋亡率明显高于空白对照组、FTY720组及吉西他滨组，差异均有统计学意义（均P<0.05）。FTY720组、吉西他滨组及联合组的A549及H520细胞内mdig表达量明显低于空白对照组［A549：（0.72±0.11）%、（0.62±0.09）%、（0.21±0.07）%比（0.86±0.12）%，H520:（0.69±0.08）%、（0.63±0.10）%、（0.18±0.11）%比（0.88±0.21）%］，且联合组mdig表达量明显低于FTY720组和吉西他滨组，差异均有统计学意义（均P<0.05）。结论    采用FTY720与吉西他滨对非小细胞肺癌细胞株处理后，可抑制细胞增殖、促进细胞凋亡并下调细胞中mdig基因表达。

• 【Abstract】Objective    To analyze the effects of FTY720 and gemcitabine on expression of oncogene mdig in non-small cell lung cancer cells. Methods    Non-small cell lung cancer cell line A549 and H720 were separately treated with FTY720, gemcitabine and FTY720＋gemcitabine; cell proliferation, apoptosis and expression of mdig were analyzed. Results    Inhibition of FTY720 on A549 and H520 cell proliferation showed dose and time dependence. Survival rates of A549 and H520 cells in FTY720＋gemcitabine group were significantly lower than those in FTY720 group and gemcitabine group［（21.8±1.0）% vs （60.1±7.3）%,（44.4±5.0）%; （23.3±1.9）% vs （43.6±2.4）%,（65.5±2.9）%］(P<0.05). Drug combination index of FTY720＋gemcitabine was lower than 1. Apoptosis rates of A549 and H520 cells in FTY720＋gemcitabine group were significantly higher than those in blank control group, FTY720 group and gemcitabine group（P<0.05）. Expression of mdig in FTY720＋gemcitabine group, FTY720 group and gemcitabine group was significantly lower than that in blank control group［A549： （0.72±0.11）%,（0.62±0.09）%,（0.21±0.07）% vs（0.86±0.12）%; H520： （0.69±0.08）%,（0.63±0.10）%,（0.18±0.11）% vs（0.88±0.21）%］; expression of mdig in FTY720＋gemcitabine group was significantly lower than that in FTY720 group and gemcitabine group（P<0.05）. Conclusion    FTY720 and gemcitabine can inhibit proliferation and promote apoptosis of non-small cell lung cancer cells and down-regulate mdig expression.