2018 年第 1 期 第 13 卷

KISS-1基因对人肺鳞状细胞癌细胞YTMLC-90增殖及侵袭能力的影响

作者：赵彬王俊然郑素琴

英文作者：

单位：063000河北省唐山市，华北理工大学校医院（赵彬），基础医学院病理学系（郑素琴）；063000河北省唐山市妇幼保健院病理科（王俊然）

英文单位：

关键词：肺肿瘤；YTMLC-90细胞；KISS-1基因；基因转染

英文关键词：

• 摘要：

• 【摘要】目的    探讨肿瘤转移抑制基因KISS-1对人肺鳞状细胞癌细胞YTMLC-90增殖及侵袭能力的影响。方法    通过基因转染技术，将携带KISS-1基因的pEGFP-N2-KISS-1质粒转染入人YTMLC-90细胞，同时设不携带KISS-1基因的空质粒转染组和空白对照组作为对照，用蛋白质印迹法检测转染后YTMLC-90细胞中KISS-1蛋白的表达以确定转染是否成功；噻唑蓝（MTT）法测定3组YTMLC-90细胞增殖情况；Millicell小室法检测3组YTMLC-90细胞的侵袭能力。结果    成功将KISS-1基因转染入YTMLC-90细胞。pEGFP-N2-KISS-1质粒转染组YTMLC-90细胞的增殖活性与空质粒转染组和空白对照组差异无统计学意义 (P>0.05)，但YTMLC-90细胞穿透Millicell聚碳酸酯膜的细胞数较空质粒转染组和空白对照组明显减少［（35±5）×105个比（82±6）×105个、（84±5）×105个］，差异有统计学意义(P<0.05)。结论    KISS-1基因对YTMLC-90细胞的增殖能力没有影响，但对其侵袭能力有抑制作用，提示KISS-1基因能够抑制肺癌的侵袭。

• Effect of KISS-1 gene on proliferation and invasion of human lung squamous carcinoma cell line YTMLC-90

  Zhao Bin, Wang Junran, Zheng Suqin

  University Hospital, North China University of Science and Technology, Hebei Province, Tangshan 063000, China（Zhao B）; Department of Pathology, School of Basic Medicine, North China University of Science and Technology, Hebei Prvince, Tangshan 063000, China（Zheng SQ）; Department of Pathology, Maternal and Child Health Care Hospital of Tangshan, Hebei Prvince, Tangshan 063000, China（Wang JR）

  Corresponding author: Zheng Suqin, Email: suqinzheng@163.com

  【Abstract】Objective     To investigate the effect of KISS-1 gene on proliferation and invasion of human lung squamous carcinoma cell line YTMLC-90. Methods    The pEGFP-N2-KISS-1 plasmid carried with KISS-1 gene and empty plasmid were respectively transfected into human YTMLC-90 cells; YTMLC-90 cells without special treatment were as a blank control group. Expression of KISS-1 protein was detected by western blot method to determine whether the transfection was successful. Proliferation activity of YTMLC-90 cells was tested by the MTT assay; invasion ability was tested by Millicell chamber method. Results    KISS-1 gene was successfully transfected into YTMLC-90 cells in pEGFP-N2-KISS-1 plasmid group. The proliferation activity in pEGFP-N2-KISS-1 plasmid group had no significant differences compared to that in empty plasmid group and blank control group（P＞0.05）. The number of cells that passed through polycarbonate membrane of Millicell chamber in pEGFP-N2-KISS-1 plasmid group was significantly less than that in empty plasmid group and blank control group［（35±5）×105 vs （82±6）×105, （84±5）×105］（P＜0.05）. Conclusion    KISS-1 gene has no effect on proliferation but inhibits invasion of YTMLC-90 cells, suggesting it may inhibit the invasion of lung cancer.

  【Key words】Lung neoplasms;YTMLC-90 cell;KISS-1 gene;Gene transfection

  【Fund program】Natural Science Foundation of Hebei Province（H2014209247）