2017 年第 10 期 第 12 卷

高尿酸血症对冠状动脉粥样硬化性心脏病大鼠炎性指标及氧化型低密度脂蛋白与凝集素样氧化型低密度脂蛋白受体1水平的影响

Effects of hyperuricemia on inflammatory factors, oxidized low-density lipoprotein and lectin-like oxidized low-density lipoprotein receptor-1 in rats with coronary atherosclerotic heart disease

作者：陆荣荣呼小龙吾麦尔江·克力木阿力木江·阿布力米提张萍瓦哈甫·马木提

英文作者：Lu Rongrong Hu Xiaolong Wumaierjiang Kelimu Alimujiang Abulimiti Zhang Ping Wahafu Mamuti

单位：844000新疆维吾尔自治区喀什地区第二人民医院心内科

英文单位：Department of Cardiology Kashgar Prefecture Second People′s Hospital Xinjiang Uygur Autonomous Region Kashi 844000 China

关键词：冠状动脉粥样硬化性心脏病；高尿酸血症；氧化型低密度脂蛋白；凝集素样氧化型低密度脂蛋白受体1

英文关键词：Coronaryatheroscleroticheartdisease;Hyperuricemia;Oxidizedlow-densitylipoprotein;Lectin-likeoxidizedlow-densitylipoproteinreceptor-1

• 摘要：

• 目的 分析高尿酸血症对冠状动脉粥样硬化性心脏病（冠心病）大鼠炎性指标及氧化型低密度脂蛋白（ox-LDL）与凝集素样氧化型低密度脂蛋白受体1（LOX-1）水平的影响。方法 选取90只清洁级健康雄性Sprague-Dawley大鼠，采用随机数字表法分为对照组、冠心病组、冠心病合并高尿酸血症组，各30只。冠心病组给予高脂肪饮食同时腹腔注射维生素D3，冠心病合并高尿酸血症组给予高脂肪饮食及尿酸酶抑制剂（氧嗪酸钾）灌胃，对照组给予0.9%氯化钠注射液腹腔注射。比较3组大鼠的C反应蛋白（CRP）、肿瘤坏死因子α（TNF-α）、基质金属蛋白酶9（MMP-9）、ox-LDL、低密度脂蛋白（LDL）及LOX-1水平，并比较3组冠状动脉内膜组织病理特征。结果 冠心病合并高尿酸血症组大鼠血清CRP、TNF-α、MMP-9、ox-LDL、LDL及LOX-1水平均明显高于冠心病组及对照组，差异均有统计学意义［（11.1±2.2）μg/L比（8.4±1.6）、（4.1±1.4）μg/L，（165±39）ng/L比（147±36）、（62±21）ng/L，（90±66）ng/L比（58±42）、（36±12）ng/L，（393±81）μg/L比（242±91）、（113±51）μg/L，（3.26±1.01）mmol/L比（2.41±0.61）、（1.05±0.41）mmol/L，（384±72）μg/L比（268±46）、（142±24）μg/L］（均P＜0.05）。冠心病合并高尿酸血症组大鼠冠状动脉内膜组织病理损伤最重，部分区域出现脂质沉积现象，泡沫细胞形成较少，有溃疡出现。结论 高尿酸血症同冠心病密切相关，血尿酸水平升高可促进炎性反应，促使ox-LDL与LOX-1进一步升高。

• Objective To investigate effects of hyperuricemia on inflammatory factors, oxidized low-density lipoprotein（ox-LDL） and lectin-like oxidized low-density lipoprotein receptor-1（LOX-1） in rats with coronary atherosclerotic heart disease（CHD）. Methods Ninety clean grade healthy male Sprague-Dawley rats were randomly divided into control group, CHD group, CHD complicated with hyperuricemia group, with 30 rats in each group. The CHD group had high fat diet and intraperitoneal injection of vitamine D3; the CHD complicated with hyperuricemia group had high fat diet and intragastric administration of uricase inhibitor potassium oxonate; the control group had intraperitoneal injection of 0.9% sodium chloride sodium. Levels of C-reactive protein（CRP）, tumor necrosis factor-α（TNF-α）, matrix metalloproteinase-9（MMP-9）, ox-LDL, LDL, LOX-1 and pathological features of coronary artery intima were analyzed. Results Levels of serum CRP, TNF-α, MMP-9, ox-LDL, LDL and LOX-1 in CHD complicated with hyperuricemia group were significantly higher than those in CHD group and control group［（11.1±2.2）μg/L vs （8.4±1.6）,（4.1±1.4）μg/L; （165±39）ng/L vs （147±36）,（62±21）ng/L; （90±66）ng/L vs （58±42）,（36±12）ng/L; （393±81）μg/L vs （242±91）,（113±51）μg/L; （3.26±1.01）mmol/L vs （2.41±0.61）,（1.05±0.41）mmol/L; （384±72）μg/L vs （268±46）,（142±24）μg/L］（P＜0.05）. Rats in CHD combined with hyperuricemia group had the most severe coronary artery intimal damage; lipid deposition was observed in some areas; foam cell formation decreased and ulceration occurred. Conclusion Hyperuricemia is closely related to CHD; elevation of serum uric acid can promote inflammatory response and increase levels of ox-LDL and LOX-1.